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Success Jaschinski et al. 1998; for more detailed discussion see Vadszov et al. 2006a ; . Nevertheless, we have performed analyses by MALDI-TOF and LC MS Ion Trap mass spectrometer techniques of rat 2x d and 2b MyHC isoforms. We have proposed new pairs of primers specific for all four MyHC isoforms designed with respect to their equal Tms and lengths of PCR products and matching to separate exons. This will make the organization of the next RT-PCR experiment more simple. In our hands, each pair of primers gave specific products under equal conditions Zurmanov et al. 2006 ; . Using our current methods, we cannot clearly demonstrate whether individual MyHC isoforms are localized in pure muscle fiber types or in transitional hybrid ; fibers containing various combinations of MyHC isoforms for review, see Pette 2002 ; . Coexpression of MyHC mRNA transcripts in a single fiber was demonstrated both in rat DeNardi et al. 1993, Stevens et al. 1999 ; and human muscles Smerdu et al. 1994, Andersen and Schiaffino 1997 ; . The recent study of muscle phenotype composition Zachaov et al. 2005 ; revealed coexpression of MyHC1 and 2a isoforms determined in serial cross sections of the SOL and of all four MyHC isoforms in the EDL muscles immunostained with specific anti MyHC monoclonal antibodies. Coexpression was especially high in the hyperthyroid SOL and hypothyroid EDL muscles Vadszov 2005 ; . Our findings suggest that as a result of chronic long-lasting alteration of thyroid status, both slow SOL and fast EDL muscles undergo characteristic changes in the expression of MyHC mRNA transcripts and MyHC protein isoforms. These changes, however, correspond to changes described after shorter intervals of thyroid status alterations. Our results also suggest that the SOL is more influenced by hyperthyroid status, whereas EDL seems to respond to both hypothyroid and hypothyroid statuses An all-natural formula containing the following ingredients: Vitamin C 200 mg Manganese 20 mg 1300 mg of the following herbs: Devil's Claw Root Extract standardized to contain 2.5% harpagosides Purple Willow Salix purpurea ; Bark Extract standardized to 6%-8% total salicin and phenylpropanolamine.

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