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I.Roine 1, A. Vasquez2, M neo1 . 1University of Chile, Santiago, Chile; 2 University Diego Portales, Santiago, Chile Introduction: RSV is a common cause of respiratory infection and hospitalization of infants. In Chile RSV produces a massive yearly epidemic with great pressure for hospital beds.We analyzed if home environment plays a role in the need for hospitalization by comparing it between ambulatory and hospitalized patients. Our setting is representative of most urban Latin America: low income, crowding, and high levels of outdoor pollution. Method: Previously healthy, term babies presenting with their first, RSV positive respiratory infection, were enrolled consecutively during 3 RSV epidemics. Patients were hospitalized if their oxygen saturation was below 95% and managed as outpatients when above 94%. Home environment was described by parents occupations, mothers educational level, family size, number of smoking family members, home contacts with a cold during the previous 7 days, kerosene heating and breastfeeding. Results: Hospitalized infants N 182 ; differed from ambulatory patients N 172 ; by having more siblings with a cold OR 1.4, CI 95% 1.1-1.7, p 0.006 ; , more smoking family members OR 1, 2, CI 95% 1.1-1.4, p 0.007 ; and not being breastfed OR 2.1, CI 95% 1.1-4.0, p 0.02 ; , but not in age or sex or the other tested variables p 0.05 ; .The number of siblings with a cold and smoking family members remained independent, significant predictors of hospitalization in logistic regression analysis. Conclusions: Indoor environment makes a difference in RSV severity!
Positive line was partly surrounding the graft. In the remaining 5% of cases, Q.Wnt-1 expression was very low and could not be precisely mapped. The reorganization of Q.Wnt-1 expression was never found in non-integrated grafts. The chick and quail Wnt-1-expressing zones, whether they were continuous 71% of cases, see Figs 5C; 6B ; or adjacent to each other 29% of cases, see Fig. 6A ; , always formed a narrow domain separating the graft from the host. ii ; In 100% of metencephalic type B ; grafts n 36 ; , Q.Wnt1 was regionalized within the grafted tissue as a more or less complete circle-shaped positive region, dorsally in contact with the host and surrounding a tissue of thin teloid appearance Figs 5H, I; 6C, D ; . Based on Wnt-1 expression in met-mesencephalon at the time of grafting see Figs 3C, D; 4G ; , this expression of Wnt-1 probably results from de novo initiation of Wnt-1 transcription within the graft. Importantly, this specific spatial organization of Wnt-1 expression was also observed in non-integrated grafts 3% of cases ; , suggesting that it could occur independently of the surrounding host. When the graft was integrated, the chick host was found to participate in the formation of a complete Wnt-1-positive circle, either through a cluster of strongly Ch.Wnt-1-positive cells on the dorsal aspect of the circle 33% of cases ; Fig. 5I ; , or through a longer line of positive cells surrounding part of the tela 67% of cases ; Fig. 5H, 6C, D ; , usually caudally. In both cases, chick and quail Wnt-1-expressing cells were in continuity with each other. iii ; Met-mesencephalic type C ; grafts n 31 ; gave more variable results concerning Wnt-1 expression, and will be detailed below, in relation with the localization of other phenotypic markers. Briefly, the two aspects described above for mesencephalic and metencephalic grafts were obtained, in various proportions depending on the exact region grafted. iv ; When portions of diencephalon or telencephalon were grafted into the same location n 12 ; , Wnt-1 expression was only observed in the graft in one case, as scattered clusters of positive cells, that is with no sign of reorganization not shown ; . All the other grafts i.e. 92% of cases ; were devoid of Wnt-1-expressing cells Fig. 5J ; . In summary, in a large majority of cases, the host and the grafted portion of met-mesencephalon were observed to cooperate to reconstruct a continuous line of Wnt-1-expressing cells delimiting territories within and around the grafted region. Identification of the territories in contact with Wnt-1positive cells during the ectopic development of met-mesencephalic regions Grafts of met-mesencephalon into chick diencephalon are able to modify the phenotype of the surrounding host tissue Gardner and Barald, 1991; Martinez et al., 1991; Bally-Cuif et al., 1992 ; . To define the phenotype of both the grafted and host structures in contact with the Wnt-1-positive cells, we used the Cotx2, Pax 6 and En-2 markers. The most frequent configurations are schematized in Fig. 8, and detailed observations are presented below. Mesencephalic type A ; grafts see Fig. 6A-B ; The grafted tissue was in all cases En-2- n 11 ; and Cotx2 n 4 ; positive, but Pax 6-negative n 14 ; , indicating that it had maintained its original mesencephalic phenotype. Cotx2 expression was not perturbed in the grafted region not shown ; . En-2 inductions were observed in the host territory in contact with the graft in 65% of cases n 11 ; see Fig. 6A, B ; , principally caudal to the graft. Pax 6 transcription was generally shut off in the induced region asterisk in Fig. 6A, B ; and replaced by En-2 expression, although regions of overlap between high expression of the two genes were sometimes observed rostrally see Fig. 6B, double arrows ; . In most 80% ; of the cases showing En-2 induction, the quail chick Wnt-1-positive line reconstructed in the periphery of the graft ran through the En2-positive territory, separating the graft from the induced host tissue see Fig. 6A, B ; . Wnt-1-positive cells were rarely found between En-2- and Pax 6-expressing domains laterally 20% of cases ; . However, the `deflected' Wnt-1-positive dorsal midline stripe joining the graft was found to border the induced tissue dorsally and to separate it from the contralateral Pax 6expressing cells in 50% of cases open arrows in Fig. 6B ; . Metencephalic type B ; grafts see Fig. 6C-D ; The grafted tissue was in most cases 71%, n 7 ; En-2 positive, although at a rather low level. No induction of En-2 in the host was ever observed around the graft. Pax 6 expression was in most cases maintained near the graft and extended caudally to the same limit as on the contralateral side not shown ; . In all cases studied n 36 ; , the dorsally located tela-like structure bordered by Wnt-1-positive cells stars in Fig. 6C, D ; showed faint Cotx2 expression only visible in Fig. 6D ; , whereas the rest of the graft appeared entirely Cotx2-negative. These two structures can therefore be identified as tela choroidea and metencephalon, respectively. In 92% of cases, Ch.Wnt-1 expression was only found to border the graft along the tela choroidea, when the latter was in contact with the host. It was rapidly interrupted as it reached Q.Wnt-1 expression and the metencephalic tissue. The latter was therefore only rarely 8% of cases ; separated from Pax 6 expression by Wnt-1-positive cells, whereas the tela choroidea was in all cases surrounded by a combination of chick and quail Wnt-1-expressing cells. Met-mesencephalic type C ; grafts Fig. 7 ; These grafts overlapped the constriction and were further subdivided in three categories depending on their rostrocaudal location: as shown in Fig. 2B, C1, C2 and C3 grafts contained approximately the same amount of tissue but a decreasing proportion of metencephalic territory. Grafts of type C1 n 10 ; , which overlap the met-mesencephalic constriction but carry a large portion of metencephalon, behaved exactly as the grafts B previously described, that is developed as a small metencephalic territory Cotx-2- ; and tela choroidea Fig. 7A ; . However, En-2 expression in the graft was generally stronger and was induced in the surrounding host in 40% of cases. In these cases, Ch.Wnt-1 expression was still found to border mainly the tela choroidea, although it could surround the whole graft in some cases Fig. 7A, small arrows ; . This time Ch.Wnt-1-positive cells were found in a En2-positive environment. Grafts of type C 2 and C3 n 21 ; Fig. 7B-D ; gave the same results. They formed in 95% of cases two different structures, separated by a small constriction within the grafted tissue. In all cases, one of these structures was Cotx2-positive and the other Cotx2-negative; their phenotype can therefore be identified as mesencephalic and metencephalic, respectively.
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Introduction: Egg activation is triggered by a rise in intracellular calcium ions Ca2 + ; . In the sea urchin egg, the Ca2 + -mobilising messengers cyclic ADP-ribose cADPR ; and nicotinic acid adenine dinucleotide phosphate NAADP ; have been implicated as playing important roles during egg activation. However, their exact functional role and mechanism of regulation remains largely unclear. The ADP-ribosyl cyclases ARCs ; are key enzymes in Ca2 + signalling as they can potentially generate both cADPR and NAADP. In this study we have identified and cloned two novel sea urchin ARCs and investigated their functional role during fertilization. Methods: By searching Strongylocentrotus purpuratus sea urchin EST and genomic databases we identified and cloned two distinct DNA sequences coding for ARC proteins, which we have named SpARC1 and SpARC2. We have used these sequences to generate SpARC-specific rabbit polyclonal antibodies. We have also expressed SpARC1 and 2 as recombinant proteins in bacteria and yeast. Endogenous ARC activity was detected as ability to synthesize cADPR from NAD and NAADP from NADP and nicotinic acid. Results & Discussion: Immmuno-blot analysis using SpARC1- and SpARC2specific antibodies confirmed their expression in S. purpuratus eggs and in particular in cell surface complexes. In these complexes, endogenous ARC enzymatic activity was detected. We investigated SpARC subcellular distribution by immunofluorescence analysis and showed that SpARC1 is on the outer vitelline layer of the egg and SpARC2 associates with cortical granules. We hypothesise that SpARCs locally synthesise second messengers where they are first needed to mobilise the Ca2 + necessary for exocytosis and fertilization envelope formation!
Table 4 Summary of Hierarchical Regression Analyses for Variables Predicting Self-efficacy Variable Warmth of Mother Warmth of Father Parent Monitoring Trust in parents Esteem from Friends Friend involvement Trust in Peers Comparison with Peers Increase in R2 Total R2 Adjusted R2 * p .05, * p .01 only two predictors significantly contribute to the 27% of the total explained variance in selfesteem. Warmth of Father keeps its importance from Step 1 and in Step 2 it is joined by Esteem from Friends. It may be concluded that the boys' self-esteem grows with perceived warmth of father and with the growing esteem or respect that the adolescent perceives from his friends. For girls, all the included predictors explain 22% of variance. Four predictors turned up significant: Warmth of Mother, Trust in Parents, Esteem from Friends, Global Comparison with Peers. The influence of trust in parents rose in the final model to the level of statistical significance. Esteem from friends and Global comparison showed as new significant predictors. All these predictors have a positive relationship with self-esteem. The results of hierarchical regression for the self-efficacy are presented in Table 4. In Step 1, parent-related variables explained significant portion of variance only for boys. For both sexes, the amount of variance in self-efficacy explained by parent-related variables was quite small 6 percent for boys and 3 percent for girls. Step 2, in which the friend and peer variables were entered, brought a significant increase in explained variance of self-efficacy in both sexes. The final patterns of significant predictors reveal some sex differences. The influence of parents is very specific. While the perceived warmth of father is positively related to boys' self-efficacy, the standardized beta coefficient for warmth of mother is not significant. Concerning the block of friend and peer variables, general comparison with peers and trust to friends are significant predictors. Girls' level of self-efficacy grows with the trust in their parents and with positive feelings received from their peers whether in the form of the feeling of acceptance or in the form of comparisons with peers. Our aim is to compare the predictive power of parent-related and friend peer-related variables. A problem can be seen in the fact that the correlations among parent-related variables in our sample are higher than the correlations among friend peer-related variables. A lower level of multicollinearity among friend peer-related variables gives them a chance to explain more variance in the dependent variable. One way to deal with this problem, though not perfect, is to aggregate the four variables for each source of influence parents vs. friends peers ; into one composite variable. Thus, we will have one score representing each adolescent's relationship with his or her parents Relationship with Parents ; and one score representing his or her relationship with friends peers Relationship with Friends Peers ; . Tables 5 and 6 present the regressions done in the same manner as those presented above with the difference that only two predictor variables were used: Relationship with Parents and Relationship with Friends Peers. Table 5 presents the results of the 2-step regression of these two variables on self-esteem and Table 6 presents their regression on self-effiacacy. The expected pattern of regression weights and explained variance did not show up in terms of statistical significance. Both aggregate variables are significant predictors of both self-esboys N 286 ; Step 1 Step 2 -.055 -.094 .236 * .214 * .029 .062 .037 * .205 * .060 * .097 * .060 .157 .041 girls N 424 ; Step 1 Step 2 .092 .049 -.015 -.107 -.067 .136 .176 * .061 -.091 .135 * .327 * .031 .134 * .031 .165 .018 and revlimid.
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With the addition of a potential therapeutic analogue in the form of pentavalent rhenium-188 186 dimercaptosuccinic acid complex [188 186 Re V ; DMSA] in the therapeutic armamentarium of nuclear medicine, 99Tcm V ; DMSA scintigraphy has been explored in recent years in various malignant conditions to predict the therapeutic efficacy of the former. In addition to its well established role in medullary thyroid carcinoma and some other soft tissue tumours, the other arena where it holds considerable promise is the targeting of metastatic bone disease. The structural similarity between 99Tcm V ; DMSA and 188Re V ; DMSA accounts for the similarity of their biodistribution [1, 2], which makes the former very useful in the estimation of tumour and renal dosimetry and thereby in selection of suitable patients for the treatment with the latter. Bone marrow depression continues to be the major dose limiting toxicity in treatment with various radiolabelled biphosphonates for pain palliation. In a recently concluded dose escalation study with 188 Re-HEDP [3], the pain palliative response rates appeared to increase with higher.
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Authorise a special serious crimes court chamber with a vetted corps of judges, prosecutors and defence counsel and permit plea-bargaining with appropriate oversight; and - provide witness protection and better pay for judges; - while donors and MINUSTAH should coordinate with the ministry of justice's national strategy and provide trainers and funding for infrastructure, witness protection, forensic capabilities and legal aid. In the longer term, the government and parliament need to: - amend the constitution to establish a more rational and effective procedure for appointing higherlevel judges; - modernise the code of criminal procedure, establish a permanent panel to review cases of lengthy pre-trial detention and expand the use of fast-track procedures for prosecution of relatively minor crimes; and - build civil society support for justice reform; - while donors and MINUSTAH should ensure their programs extend for at least five years and, together with the government and other members of the Caribbean Community and Common Market Caricom ; , should create a hybrid court with Haitian and other judges and personnel from the region to try transnational crime cases. Port-au-Prince Brussels, 31 January 2007 Full Report pdf * format - 180.5 Kbytes and rhinocort.
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The effects of human serum on the MIC and MBC of BMS-284756 were determined using two isolates each of group A -haemolytic streptococci, Moraxella catarrhalis, MSSA, S. pneumoniae and E. coli. A microdilution technique, as described previously, 9 was employed using IsoSensitest broth Oxoid, Basingstoke, UK ; plus 0, 20 or 70% human serum TCS, Bodolph Clayton, UK ; and supplemented with 5% laked horse blood Oxoid ; for fastidious bacteria. Doubling dilutions of BMS-284756 were inoculated with 105 cfu mL of the test organism and incubated as previously. The MIC was defined as above, the minimal bactericidal concentration MBC ; as the lowest concentration of antimicrobial to prevent growth on subculture of the broth for 1824 h in appropriate conditions and rhogam.
Fig. 12. Transport function of rROAT1-GFP in transfected killifish renal proximal tubules. Two days after transfection with either pEGFP-C3 or pEGFP-C3 rROAT1, tubules were exposed to 1 M their culture medium. After 30 min, confocal images were acquired, and cellular and luminal fluorescence intensities were measured from the stored confocal images. Each bar represents mean fluorescence intensity for 912 tubules SE. Cellular and luminal fluorescence intensities were significantly greater in pEGFP-C3 rROAT1-transfected tubules than in pEGFP-C3-transfected tubules * P 0.05 and * P 0.0001, respectively ; . Transport assay was performed on 3 separate occasions, and a representative experiment is shown. Fig. 10. Cross-sectional image analysis of rROAT1-GFP-expressing killifish renal proximal tubules. Image stack was obtained as described in Fig. 4. A: pEGFP-C3 rROAT1-transfected tubule. Lines b and c designate the axes used for cross sections through the image stack shown in B and C, respectively ; . Note strong signal in basal and lateral membranes of tubule cells and absence of signal from nucleus and apical region and restasis
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